| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RANGAP1 (398-587) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RANGAP1 |
| Gene ID: |
5905 |
| Uniprot ID: |
RAGP1_HUMAN |
| Immunogen Region: |
398-587 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 398-587 of human RanGAP1 (NP_002874.1). |
| Immunogen Sequence: |
PQQRGQGEKSATPSRKILDP NTGEPAPVLSSPPPADVSTF LAFPSPEKLLRLGPKSSVLI AQQTDTSDPEKVVSAFLKVS SVFKDEATVRMAVQDAVDAL MQKAFNSSSFNSNTFLTRLL VHMGLLKSEDKVKAIANLYG PLMALNHMVQQDYFPKALAP LLLAFVTKPNSALESCSFAR HSLLQTLYKV |
| Tissue Specificity | Highly expressed in brain, thymus and testis. |
| Post Translational Modifications | Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBE2I. Desumoylated by HINT1. |
| Function | GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export. |
| Protein Name | Ran Gtpase-Activating Protein 1Rangap1 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-165054Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-4615885Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9615933Reactome: R-HSA-9648025Reactome: R-HSA-9793242 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmNucleus EnvelopeChromosomeCentromereKinetochoreCytoskeletonSpindleCytoplasmic During InterphaseDetected At The Nuclear Envelope During InterphaseTargeted To The Nuclear Pores After SumoylationDuring MitosisAssociates With Mitotic SpindlesBut Is Essentially Not Detected At The Spindle PolesAssociation With Kinetochores Appears Soon After Nuclear Envelope Breakdown And Persists Until Late AnaphaseMitotic Location Also Requires Sumoylation |
| Alternative Antibody Names | Anti-Ran Gtpase-Activating Protein 1 antibodyAnti-Rangap1 antibodyAnti-RANGAP1 antibodyAnti-KIAA1835 antibodyAnti-SD antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
