Western blot analysis of various lysates using RAI14 Rabbit polyclonal antibody (STJ118256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RAI14 antibody (300-600) (STJ118256)

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STJ118256
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAI14
Gene ID: 26064
Uniprot ID: RAI14_HUMAN
Immunogen Region: 300-600
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human RAI14 (NP_056392.2).
Immunogen Sequence: SGKESVFFAEPPFKAEISSI RENKDRLSDSTTGADSLLDI SSEADQQDLLSLLQAKVASL TLHNKELQDKLQAKSPKEAE ADLSFDSYHSTQTDLGPSLG KPGETSPPDSKSSPSVLIHS LGKSTTDNDVRIQQLQEILQ DLQKRLESSEAERKQLQVEL QSRRAELVCLNNTEISENSS DLSQKLKETQSKYEEAMKEV LSVQKQMKLGLVSPESMDNY SHFHELRVTEEEINVLKQD
Tissue Specificity Highly expressed in placenta, muscle, kidney and testis. Moderately expressed in heart, brain, lung, liver and intestine. Isoform 2 is widely expressed and expressed in fetal and adult testes, and spermatozoa.
Function Plays a role in actin regulation at the ectoplasmic specialization, a type of cell junction specific to testis. Important for establishment of sperm polarity and normal spermatid adhesion. May also promote integrity of Sertoli cell tight junctions at the blood-testis barrier.
Protein Name Ankycorbin
Ankyrin Repeat And Coiled-Coil Structure-Containing Protein
Novel Retinal Pigment Epithelial Cell Protein
Retinoic Acid-Induced Protein 14
Cellular Localisation Cytoplasm
Cytoskeleton
Stress Fiber
Cell Cortex
Cell Junction
Nucleus
Associated With The Cortical Actin Cytoskeleton Structures In Terminal Web And Cell-Cell Adhesion Sites
Highly Expressed At The Ectoplasmic Specialization
An Actin-Rich Cell Junction Specific To The Testis
Predominantly Nuclear In Nonconfluent Cells
Alternative Antibody Names Anti-Ankycorbin antibody
Anti-Ankyrin Repeat And Coiled-Coil Structure-Containing Protein antibody
Anti-Novel Retinal Pigment Epithelial Cell Protein antibody
Anti-Retinoic Acid-Induced Protein 14 antibody
Anti-RAI14 antibody
Anti-KIAA1334 antibody
Anti-NORPEG antibody

Information sourced from Uniprot.org

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