| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAD51C (1-135) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAD51C |
| Gene ID: |
5889 |
| Uniprot ID: |
RA51C_HUMAN |
| Immunogen Region: |
1-135 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human RAD51C (NP_002867.1). |
| Immunogen Sequence: |
MRGKTFRFEMQRDLVSFPLS PAVRVKLVSAGFQTAEELLE VKPSELSKEVGISKAEALET LQIIRRECLTNKPRYAGTSE SHKKCTALELLEQEHTQGFI ITFCSALDDILGGGVPLMKT TEICGAPGVGKTQLW |
| Tissue Specificity | Expressed in a variety of tissues, with highest expression in testis, heart muscle, spleen and prostate. |
| Function | Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Part of the RAD51 paralog protein complexes BCDX2 and CX3 which act at different stages of the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 seems to act downstream of BRCA2 recruitment and upstream of RAD51 recruitment.CX3 seems to act downstream of RAD51 recruitment.both complexes bind predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. Involved in RAD51 foci formation in response to DNA damage suggesting an involvement in early stages of HR probably in the invasion step. Has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Participates in branch migration and HJ resolution and thus is important for processing HR intermediates late in the DNA repair process.the function may be linked to the CX3 complex. Part of a PALB2-scaffolded HR complex containing BRCA2 and which is thought to play a role in DNA repair by HR. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis. |
| Protein Name | Dna Repair Protein Rad51 Homolog 3R51h3Rad51 Homolog CRad51-Like Protein 2 |
| Database Links | Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693616Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709603Reactome: R-HSA-983231 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMitochondrionDna Damage Induces An Increase In Nuclear LevelsAccumulates In Dna Damage Induced Nuclear Foci Or Rad51c Foci Which Is Formed During The S Or G2 Phase Of Cell CycleAccumulation At Dna Lesions Requires The Presence Of Nbn/Nbs1Atm And Rpa |
| Alternative Antibody Names | Anti-Dna Repair Protein Rad51 Homolog 3 antibodyAnti-R51h3 antibodyAnti-Rad51 Homolog C antibodyAnti-Rad51-Like Protein 2 antibodyAnti-RAD51C antibodyAnti-RAD51L2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
