| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Rad51 (1-339) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAD51 |
| Gene ID: |
5888 |
| Uniprot ID: |
RAD51_HUMAN |
| Immunogen Region: |
1-339 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-339 of human Rad51 (NP_002866.2). |
| Immunogen Sequence: |
MAMQMQLEANADTSVEEESF GPQPISRLEQCGINANDVKK LEEAGFHTVEAVAYAPKKEL INIKGISEAKADKILAEAAK LVPMGFTTATEFHQRRSEII QITTGSKELDKLLQGGIETG SITEMFGEFRTGKTQICHTL AVTCQLPIDRGGGEGKAMYI DTEGTFRPERLLAVAERYGL SGSDVLDNVAYARAFNTDHQ TQLLYQASAMMVESRYALLI VDSATALYRTDYSGRGELS |
| Tissue Specificity | Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast. |
| Post Translational Modifications | Ubiquitinated by the SCF(FBH1) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA. Ubiquitinated by RFWD3 in response to DNA damage: ubiquitination leads to degradation by the proteasome, promoting homologous recombination. Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function. |
| Function | Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair. |
| Protein Name | Dna Repair Protein Rad51 Homolog 1Hsrad51Hrad51Rad51 Homolog A |
| Database Links | Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693616Reactome: R-HSA-8953750Reactome: R-HSA-912446Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionMitochondrion MatrixChromosomeCytoskeletonMicrotubule Organizing CenterCentrosomeColocalizes With Rad51ap1 And Rpa2 To Multiple Nuclear Foci Upon Induction Of Dna DamageDna Damage Induces An Increase In Nuclear LevelsTogether With Fignl1Redistributed In Discrete Nuclear Dna Damage-Induced Foci After Ionizing Radiation (Ir) Or Camptothecin (Cpt) TreatmentAccumulated At Sites Of Dna Damage In A Spidr-Dependent MannerRecruited At Sites Of Dna Damage In A Mcm9-Mcm8-Dependent MannerColocalizes With Ercc5/Xpg To Nuclear Foci In S PhaseRecruited To Stalled Replication Forks During Replication Stress By The Tonsl-Mms22l ComplexAs Well As Atad5 And Wdr48 In An Atr-Dependent Manner |
| Alternative Antibody Names | Anti-Dna Repair Protein Rad51 Homolog 1 antibodyAnti-Hsrad51 antibodyAnti-Hrad51 antibodyAnti-Rad51 Homolog A antibodyAnti-RAD51 antibodyAnti-RAD51A antibodyAnti-RECA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
