| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Double-strand-break repair protein rad21 homolog (521-570 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAD21 |
| Gene ID: |
5885 |
| Uniprot ID: |
RAD21_HUMAN |
| Immunogen Region: |
521-570 aa |
| Specificity: |
Rad21 Polyclonal Antibody detects endogenous levels of Rad21 protein. |
| Immunogen: |
The antiserum was produced against synthesized peptide derived from the human RAD21 at the amino acid range 521-570 |
| Post Translational Modifications | Cleaved by separase/ESPL1 at the onset of anaphase.this cleavage is required for sister chromatid separation and cytokinesis. Cleaved by caspase-3/CASP3 or caspase-7/CASP7 at the beginning of apoptosis. Phosphorylated.becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK1. |
| Function | Double-strand-break repair protein rad21 homolog: As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome. May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter. May play a role in embryonic gut development, possibly through the regulation of enteric neuron development. 64-kDa C-terminal product: May promote apoptosis. |
| Protein Name | Double-Strand-Break Repair Protein Rad21 HomologHhr21Nuclear Matrix Protein 1Nxp-1Scc1 Homolog Cleaved Into - 64-Kda C-Terminal Product64-Kda Carboxy-Terminal Product65-Kda Carboxy-Terminal Product |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-2467813Reactome: R-HSA-2468052Reactome: R-HSA-2470946Reactome: R-HSA-2500257Reactome: R-HSA-3108214Reactome: R-HSA-9018519 |
| Cellular Localisation | Double-Strand-Break Repair Protein Rad21 Homolog: NucleusNucleus MatrixChromosomeCentromereCytoplasmCytoskeletonSpindle PoleAssociates With ChromatinBefore ProphaseScattered Along Chromosome ArmsDuring Prophase And PrometaphaseMost Cohesins Dissociate From The Arms Of Condensing ChromosomePossibly Through Plk1-Mediated PhosphorylationA Small Amount Of Cohesin Remains In Centromeric Regions And Is Removed From Chromosomes Only At The Onset Of AnaphaseAt AnaphaseCleavage By Separase/Espl1 Leads To The Dissociation Of Cohesin From Chromosomes And Chromosome Separation64-Kda C-Terminal Product: CytoplasmCytosolNucleus |
| Alternative Antibody Names | Anti-Double-Strand-Break Repair Protein Rad21 Homolog antibodyAnti-Hhr21 antibodyAnti-Nuclear Matrix Protein 1 antibodyAnti-Nxp-1 antibodyAnti-Scc1 Homolog Cleaved Into - 64-Kda C-Terminal Product antibodyAnti-64-Kda Carboxy-Terminal Product antibodyAnti-65-Kda Carboxy-Terminal Product antibodyAnti-RAD21 antibodyAnti-HR21 antibodyAnti-KIAA0078 antibodyAnti-NXP1 antibodyAnti-SCC1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
