• Immunoprecipitation analysis of 200 Mu g extracts of K562 cells using 1 Mu g RAD17 antibody (STJ27312). Western blot was performed from the immunoprecipitate using RAD17 antibody (STJ27312) at a dilution of 1:1000.
  • Western blot analysis of various lysates using RAD17 Rabbit polyclonal antibody (STJ27312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-RAD17 antibody (391-670) (STJ27312)

SKU:
STJ27312

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAD17 (391-670) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAD17
Gene ID: 5884
Uniprot ID: RAD17_HUMAN
Immunogen Region: 391-670
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1).
Immunogen Sequence: KILYCKRASLTELDSPRLPS HLSEYERDTLLVEPEEVVEM SHMPGDLFNLYLHQNYIDFF MEIDDIVRASEFLSFADILS GDWNTRSLLREYSTSIATRG VMHSNKARGYAHCQGGGSSF RPLHKPQWFLINKKYRENCL AAKALFPDFCLPALCLQTQL LPYLALLTIPMRNQAQISFI QDIGRLPLKRHFGRLKMEAL TDREHGMIDPDSGDEAQLNG GHSAEESLGEPTQATVPET
Tissue Specificity Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors).
Post Translational Modifications Phosphorylated. Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4.
Function Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the RAD1-RAD9-HUS1 complex and RHNO1 onto chromatin, and in CHEK1 activation. May also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Protein Name Cell Cycle Checkpoint Protein Rad17
Hrad17
Rf-C/Activator 1 Homolog
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-5685938
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9709570
Cellular Localisation Nucleus
Phosphorylated Form Redistributes To Discrete Nuclear Foci Upon Dna Damage
Alternative Antibody Names Anti-Cell Cycle Checkpoint Protein Rad17 antibody
Anti-Hrad17 antibody
Anti-Rf-C/Activator 1 Homolog antibody
Anti-RAD17 antibody
Anti-R24L antibody

Information sourced from Uniprot.org

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