| Host: |
Rabbit |
| Applications: |
WB/IP/ELISA |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAD17 |
| Gene ID: |
5884 |
| Uniprot ID: |
RAD17_HUMAN |
| Immunogen Region: |
391-670 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 391-670 of human RAD17 (NP_002864.1). |
| Immunogen Sequence: |
KILYCKRASLTELDSPRLPS HLSEYERDTLLVEPEEVVEM SHMPGDLFNLYLHQNYIDFF MEIDDIVRASEFLSFADILS GDWNTRSLLREYSTSIATRG VMHSNKARGYAHCQGGGSSF RPLHKPQWFLINKKYRENCL AAKALFPDFCLPALCLQTQL LPYLALLTIPMRNQAQISFI QDIGRLPLKRHFGRLKMEAL TDREHGMIDPDSGDEAQLNG GHSAEESLGEPTQATVPET |
| Tissue Specificity | Overexpressed in various cancer cell lines and in colon carcinoma (at protein level). Isoform 2 and isoform 3 are the most abundant isoforms in non irradiated cells (at protein level). Ubiquitous at low levels. Highly expressed in testis, where it is expressed within the germinal epithelium of the seminiferous tubuli. Weakly expressed in seminomas (testicular tumors). |
| Post Translational Modifications | Phosphorylation on Ser-646 and Ser-656 is cell cycle-regulated, enhanced by genotoxic stress, and required for activation of checkpoint signaling. Phosphorylation is mediated by ATR upon UV or replication arrest, whereas it may be mediated both by ATR and ATM upon ionizing radiation. Phosphorylation on both sites is required for interaction with RAD1 but dispensable for interaction with RFC3 or RFC4. Phosphorylation at Thr-633 by ATM in response to DNA damage promotes interaction with NBN and recruitment of the MRN complex to DNA damage sites. |
| Function | Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Has a weak ATPase activity required for binding to chromatin. Participates in the recruitment of the 9-1-1 (RAD1-RAD9-HUS1) complex and RHNO1 onto chromatin, and in CHEK1 activation. Involved in homologous recombination by mediating recruitment of the MRN complex to DNA damage sites. May also serve as a sensor of DNA replication progression. |
| Protein Name | Cell Cycle Checkpoint Protein Rad17Hrad17Rf-C/Activator 1 Homolog |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | NucleusChromosomePhosphorylated Form Redistributes To Discrete Nuclear Foci Upon Dna DamageLocalizes To Dna Double-Strand Breaks (Dsbs) |
| Alternative Antibody Names | Anti-Cell Cycle Checkpoint Protein Rad17 antibodyAnti-Hrad17 antibodyAnti-Rf-C/Activator 1 Homolog antibodyAnti-RAD17 antibodyAnti-R24L antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
