| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Rat/Human/Mouse/Non-Human Primates/Xenopus |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Rab 3 (N-Term) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol. |
| Purification: |
This antibody was antigen affinity purified from pooled serum. |
| Dilution Range: |
WB 1:1000IHCICCIP |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Rab3a |
| Gene ID: |
25531 |
| Uniprot ID: |
RAB3A_RAT |
| Immunogen Region: |
N-Term |
| Immunogen: |
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. |
| Tissue Specificity | Detected in brain. |
| Post Translational Modifications | Phosphorylation of Thr-86 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM and RAB GDP dissociation inhibitors GDI1 and GDI2. |
| Function | Small GTP-binding protein that plays a central role in regulated exocytosis and secretion. Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane. Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane. Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed. Stimulates insulin secretion through interaction with RIMS2 and RPH3AL effectors in pancreatic beta cells. Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9. Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1. Plays a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT. Interacts with MADD (via uDENN domain).the GTP-bound form is preferred for interaction. |
| Protein Name | Ras-Related Protein Rab-3a |
| Database Links | Reactome: R-RNO-181429Reactome: -RNO-181430Reactome: -RNO-210500Reactome: -RNO-212676Reactome: -RNO-264642Reactome: -RNO-6798695Reactome: -RNO-8873719Reactome: -RNO-8876198Reactome: -RNO-888590 |
| Cellular Localisation | CytoplasmCytosolLysosomeCytoplasmic VesicleSecretory VesicleCell ProjectionAxonCell MembraneLipid-AnchorCytoplasmic SidePresynapsePostsynapseCycles Between A Vesicle-Associated Gtp-Bound Form And A Cytosolic Gdp-Bound Form |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-3a antibodyAnti-Rab3a antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
