| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAB27A is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. Use at a dilution of 1:300 to 1: 2000. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
RAB27A |
| Gene ID: |
5873 |
| Uniprot ID: |
RB27A_HUMAN |
| Specificity: |
Specific for RAB27. |
| Immunogen: |
A synthetic peptide from human RAB27A conjugated to blue carrier protein has been used as the antigen. |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate homeostasis of late endocytic pathway, including endosomal positioning, maturation and secretion. Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. |
| Protein Name | Ras-Related Protein Rab-27aRab-27Gtp-Binding Protein Ram |
| Database Links | Reactome: R-HSA-264876Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9824585 |
| Cellular Localisation | MembraneLipid-AnchorMelanosomeLate EndosomeLysosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To Endosomal Exocytic Vesicles |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-27a antibodyAnti-Rab-27 antibodyAnti-Gtp-Binding Protein Ram antibodyAnti-RAB27A antibodyAnti-RAB27 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of HepG2 cells using [KD Validated] RAB27A Rabbit polyclonal antibody (STJ25258) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/100398/386820/STJ25258_1__87091.1713155968.jpg?c=1 "Immunofluorescence analysis of HepG2 cells using [KD Validated] RAB27A Rabbit polyclonal antibody (STJ25258) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
