• Western blot analysis of various lysates, using RAB11FIP5 rabbit polyclonal antibody (STJ11100112) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates, using RAB11FIP5 rabbit polyclonal antibody (STJ11100112) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of A-431 cells using RAB11FIP5 rabbit polyclonal antibody (STJ11100112) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using RAB11FIP5 rabbit polyclonal antibody (STJ11100112) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RAB11FIP5 antibody (354-653) (STJ11100112)

SKU:
STJ11100112

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB11FIP5 (354-653) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB11FIP5
Gene ID: 26056
Uniprot ID: RFIP5_HUMAN
Immunogen Region: 354-653
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 354-653 of human RAB11FIP5 (NP_056285.1).
Immunogen Sequence: HRSSISGSLPSSGSLQAVSS RFSEEGPRSTDDTWPRGSRS NSSSEAVLGQEELSAQAKVL APGASHPGEEEGARLPEGKP VQVATPIVASSEAVAEKEGA RKEERKPRMGLFHHHHQGLS RSELGRRSSLGEKGGPILGA SPHHSSSGEEKAKSSWFGLR EAKDPTQKPSPHPVKPLSAA PVEGSPDRKQSRSSLSIALS SGLEKLKTVTSGSIQPVTQA PQAGQMVDTKRLKDSAVLD
Tissue Specificity Detected at low levels in heart, brain, placenta, lung, liver, adipocytes, kidney, spleen, skeletal muscle and pancreas.
Post Translational Modifications Phosphorylated on serine and threonine residues. Phosphorylation at Ser-357 is PKA-dependent.
Function Rab effector involved in protein trafficking from apical recycling endosomes to the apical plasma membrane. Involved in insulin granule exocytosis. May regulate V-ATPase intracellular transport in response to extracellular acidosis.
Protein Name Rab11 Family-Interacting Protein 5
Rab11-Fip5
Gamma-Snap-Associated Factor 1
Gaf-1
Phosphoprotein Pp75
Rab11-Interacting Protein Rip11
Cellular Localisation Cytoplasm
Recycling Endosome Membrane
Peripheral Membrane Protein
Early Endosome Membrane
Golgi Apparatus Membrane
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Mitochondrion Membrane
Alternative Antibody Names Anti-Rab11 Family-Interacting Protein 5 antibody
Anti-Rab11-Fip5 antibody
Anti-Gamma-Snap-Associated Factor 1 antibody
Anti-Gaf-1 antibody
Anti-Phosphoprotein Pp75 antibody
Anti-Rab11-Interacting Protein Rip11 antibody
Anti-RAB11FIP5 antibody
Anti-GAF1 antibody
Anti-KIAA0857 antibody
Anti-RIP11 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance