| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | QARS1 |
| Gene ID: | 5859 |
| Uniprot ID: | SYQ_HUMAN |
| Immunogen Region: | 1-250 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human QARS (NP_005042.1). |
| Immunogen Sequence: | MAALDSLSLFTSLGLSEQKA RETLKNSALSAQLREAATQA QQTLGSTIDKATGILLYGLA SRLRDTRRLSFLVSYIASKK IHTEPQLSAALEYVRSHPLD PIDTVDFERECGVGVIVTPE QIEEAVEAAINRHRPQLLVE RYHFNMGLLMGEARAVLKWA DGKMIKNEVDMQVLHLLGPK LEADLEKKFKVAKARLEETD RRTAKDVVENGETADQTLSL MEQLRGEALKFHKPGENYK |
| Tissue Specificity | Highly expressed in fetal cerebral cortex, particularly in the ventricular zone, inner subventricular zone, outer subventricular zone, and cortical plate. |
| Function | Glutamine--tRNA ligase. Plays a critical role in brain development. |
| Protein Name | Glutamine--Trna LigaseGlutaminyl-Trna SynthetaseGlnrs |
| Database Links | Reactome: R-HSA-2408522Reactome: R-HSA-379716Reactome: R-HSA-379726Reactome: R-HSA-9856649 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Glutamine--Trna Ligase antibodyAnti-Glutaminyl-Trna Synthetase antibodyAnti-Glnrs antibodyAnti-QARS1 antibodyAnti-QARS antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ABCF2 knockout (KO) 293T cells, using [KO Validated] ABCF2 Rabbit polyclonal antibody (STJ11100826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89762/358237/STJ11100826_1__74130.1713122569.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ABCF2 knockout (KO) 293T cells, using [KO Validated] ABCF2 Rabbit polyclonal antibody (STJ11100826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western blot analysis of lysates from wild type (WT) and Annexin A6/Annexin VI knockout (KO) HeLa cells, using [KO Validated] Annexin A6/Annexin VI Rabbit polyclonal antibody (STJ11100042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89114/357566/STJ11100042_1__16062.1713121692.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Annexin A6/Annexin VI knockout (KO) HeLa cells, using [KO Validated] Annexin A6/Annexin VI Rabbit polyclonal antibody (STJ11100042) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
