| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PYGL |
| Gene ID: | 5836 |
| Uniprot ID: | PYGL_HUMAN |
| Immunogen Region: | 690-847 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 690-847 of human PYGL (NP_002854.3). |
| Immunogen Sequence: | IGTMDGANVEMAEEAGEENL FIFGMRIDDVAALDKKGYEA KEYYEALPELKLVIDQIDNG FFSPKQPDLFKDIINMLFYH DRFKVFADYEAYVKCQDKVS QLYMNPKAWNTMVLKNIAAS GKFSSDRTIKEYAQNIWNVE PSDLKISLSNESNKVNGN |
| Post Translational Modifications | Acetylation, which is up-regulated by glucose and insulin and down-regulated by glucagon, inhibits the glycogen phosphorylase activity by promoting PPP1R3B-mediated recruitment of phosphatase PP1 and Ser-15 dephosphorylation. Phosphorylation at Ser-15 converts inactive phosphorylase b into active phosphorylase a. Dephosphorylation of Ser-15 by phosphatase PP1 inactivates the enzyme. |
| Function | Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis. |
| Protein Name | Glycogen Phosphorylase - Liver Form |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70221 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Antibody Names | Anti-Glycogen Phosphorylase - Liver Form antibodyAnti-PYGL antibody |
Information sourced from Uniprot.org