| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PXDN (1320-1479) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PXDN |
| Gene ID: |
7837 |
| Uniprot ID: |
PXDN_HUMAN |
| Immunogen Region: |
1320-1479 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1320-1479 of human PXDN (NP_036425.1). |
| Immunogen Sequence: |
RTRGQFNAFSYHFRGRRSLE FSYQEDKPTKKTRPRKIPSV GRQGEHLSNSTSAFSTRSDA SGTNDFREFVLEMQKTITDL RTQIKKLESRLSTTECVDAG GESHANNTKWKKDACTICEC KDGQVTCFVEACPPATCAVP VNIPGACCPVCLQKRAEEKP |
| Tissue Specificity | Expressed at higher levels in heart, lung, ovary, spleen, intestine and placenta, and at lower levels in liver, colon, pancreas, kidney, thymus, skeletal muscle and prostate. Expressed in tumors such as melanoma, breast cancer, ovarian cancer and glioblastoma. A shorter form probably lacking the signal sequence is found in testis and in EB1 cells undergoing p53/TP53-dependent apoptosis. |
| Post Translational Modifications | Glycosylated. Four sites are completely N-glycosylated (Asn-640, Asn-731, Asn-865 and Asn-1425), whereas the others are found partially glycosylated. Processed by FURIN and the proteolytic processing largely depends on the peroxidase activity of PXDN. The proteolytic cleavage occurs after intracellular homotrimerization and releases into the extracellular matrix a large, catalytically active fragment and a smaller fragment consisting primarily of the C-terminal VWFC domain. The processing enhances both peroxidase activity and sulfilimine cross-links formation. |
| Function | Catalyzes the two-electron oxidation of bromide by hydrogen peroxide and generates hypobromite as a reactive intermediate which mediates the formation of sulfilimine cross-links between methionine and hydroxylysine residues within an uncross-linked collagen IV/COL4A1 NC1 hexamer. In turns, directly contributes to the collagen IV network-dependent fibronectin/FN and laminin assembly, which is required for full extracellular matrix (ECM)-mediated signaling. Thus, sulfilimine cross-links are essential for growth factor-induced cell proliferation and survival in endothelial cells, an event essential to basement membrane integrity. In addition, through the bromide oxidation, may promote tubulogenesis and induce angiogenesis through ERK1/2, Akt, and FAK pathways. Moreover brominates alpha2 collagen IV chain/COL4A2 at 'Tyr-1485' and leads to bromine enrichment of the basement membranes. In vitro, can also catalyze the two-electron oxidation of thiocyanate and iodide and these two substrates could effectively compete with bromide and thus inhibit the formation of sulfilimine bonds. Binds laminins. May play a role in the organization of eyeball structure and lens development during eye development. |
| Protein Name | Peroxidasin HomologMelanoma-Associated Antigen Mg50Peroxidasin 1Hspxd01Vascular Peroxidase 1P53-Responsive Gene 2 Protein Cleaved Into - Pxdn Active Fragment |
| Database Links | Reactome: R-HSA-2243919 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixEndoplasmic ReticulumCell SurfaceBasement MembraneEnriched In The Peritubular Space Of Fibrotic KidneysAdheres On The Cell Surface In 'Hot Spots'Only The Proteolytically Processed Pxdn Integrates Into The Extracellular MatrixPxdn Active Fragment: Secreted |
| Alternative Antibody Names | Anti-Peroxidasin Homolog antibodyAnti-Melanoma-Associated Antigen Mg50 antibodyAnti-Peroxidasin 1 antibodyAnti-Hspxd01 antibodyAnti-Vascular Peroxidase 1 antibodyAnti-P53-Responsive Gene 2 Protein Cleaved Into - Pxdn Active Fragment antibodyAnti-PXDN antibodyAnti-KIAA0230 antibodyAnti-MG50 antibodyAnti-PRG2 antibodyAnti-PXD01 antibodyAnti-VPO antibodyAnti-VPO1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
