| Host: |
Rabbit |
| Applications: |
WB/IP/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PUF60 |
| Gene ID: |
22827 |
| Uniprot ID: |
PUF60_HUMAN |
| Immunogen Region: |
243-542 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 243-542 of human PUF60 (NP_055096.2). |
| Immunogen Sequence: |
DPTTGKHKGYGFIEYEKAQS SQDAVSSMNLFDLGGQYLRV GKAVTPPMPLLTPATPGGLP PAAAVAAAAATAKITAQEAV AGAAVLGTLGTPGLVSPALT LAQPLGTLPQAVMAAQAPGV ITGVTPARPPIPVTIPSVGV VNPILASPPTLGLLEPKKEK EEEELFPESERPEMLSEQEH MSISGSSARHMVMQKLLRKQ ESTVMVLRNMVDPKDIDDDL EGEVTEECGKFGAVNRVII |
| Tissue Specificity | Isoform 2 is expressed in colonic epithelium and colorectal epithelium cancer (at protein level). Isoform 6 is expressed in colorectal epithelial cancer but below detection level in colonic epithelium. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. |
| Function | DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA.binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA. |
| Protein Name | Poly(U-Binding-Splicing Factor Puf6060 Kda Poly(U-Binding-Splicing FactorFuse-Binding Protein-Interacting RepressorFbp-Interacting RepressorRo-Binding Protein 1Robp1Siah-Binding Protein 1Siah-Bp1 |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusColocalizes Partially With Ro60 |
| Alternative Antibody Names | Anti-Poly(U-Binding-Splicing Factor Puf60 antibodyAnti-60 Kda Poly(U-Binding-Splicing Factor antibodyAnti-Fuse-Binding Protein-Interacting Repressor antibodyAnti-Fbp-Interacting Repressor antibodyAnti-Ro-Binding Protein 1 antibodyAnti-Robp1 antibodyAnti-Siah-Binding Protein 1 antibodyAnti-Siah-Bp1 antibodyAnti-PUF60 antibodyAnti-FIR antibodyAnti-ROBPI antibodyAnti-SIAHBP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
