• Immunofluorescence analysis of SH-SY5Y cells using PTPN5 Rabbit polyclonal antibody (STJ119012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Neuro-2a cells using PTPN5 Rabbit polyclonal antibody (STJ119012) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PTPN5 antibody (STJ119012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PTPN5 antibody (190-307) (STJ119012)

SKU:
STJ119012

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPN5
Gene ID: 84867
Uniprot ID: PTN5_HUMAN
Immunogen Region: 190-307
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 190-307 of human PTPN5 (NP_116170.3).
Immunogen Sequence: SFTYSEWMEEKIEDDFLDLD PVPETPVFDCVMDIKPEADP TSLTVKSMGLQERRGSNVSL TLDMCTPGCNEEGFGYLMSP REESAREYLLSASRVLQAEE LHEKALDPFLLQAEFFEI
Post Translational Modifications Phosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.
Function May regulate the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
Protein Name Tyrosine-Protein Phosphatase Non-Receptor Type 5
Neural-Specific Protein-Tyrosine Phosphatase
Striatum-Enriched Protein-Tyrosine Phosphatase
Step
Database Links Reactome: R-HSA-9008059
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 5 antibody
Anti-Neural-Specific Protein-Tyrosine Phosphatase antibody
Anti-Striatum-Enriched Protein-Tyrosine Phosphatase antibody
Anti-Step antibody
Anti-PTPN5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance