| Short Description : | Rabbit polyclonal PTPN4 (1-240) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PTPN4 |
| Gene ID: | 5775 |
| Uniprot ID: | PTN4_HUMAN |
| Immunogen Region: | 1-240 |
| Immunogen Sequence: | MTSRFRLPAGRTYNVRASEL ARDRQHTEVVCNILLLDNTV QAFKVNKHDQGQVLLDVVFK HLDLTEQDYFGLQLADDSTD NPRWLDPNKPIRKQLKRGSP YSLNFRVKFFVSDPNKLQEE YTRYQYFLQIKQDILTGRLP CPSNTAALLASFAVQSELGD YDQSENLSGYLSDYSFIPNQ PQDFEKEIAKLHQQHIGLSP AEAEFNYLNTARTLELYGVE FHYARDQSNNEIMIGVMSG |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PTPN4 (NP_002821.1). |
| Post Translational Modifications | Highly phosphorylated on serine and threonine residues but not on tyrosines. Cleaved and activated by calpain I/CAPN1. |
| Function | Phosphatase that plays a role in immunity, learning, synaptic plasticity or cell homeostasis. Regulates neuronal cell homeostasis by protecting neurons against apoptosis. Negatively regulates TLR4-induced interferon beta production by dephosphorylating adapter TICAM2 and inhibiting subsequent TRAM-TRIF interaction. Also dephosphorylates the immunoreceptor tyrosine-based activation motifs/ITAMs of the TCR zeta subunit and thereby negatively regulates TCR-mediated signaling pathway. May act at junctions between the membrane and the cytoskeleton. |
| Protein Name | Tyrosine-Protein Phosphatase Non-Receptor Type 4Protein-Tyrosine Phosphatase Meg1MegPtpase-Meg1 |
| Database Links | Reactome: R-HSA-166016Reactome: R-HSA-9008059Reactome: R-HSA-9022699 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmCytoskeleton |
| Alternative Antibody Names | Anti-Tyrosine-Protein Phosphatase Non-Receptor Type 4 antibodyAnti-Protein-Tyrosine Phosphatase Meg1 antibodyAnti-Meg antibodyAnti-Ptpase-Meg1 antibodyAnti-PTPN4 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MAPKAPK3 knockout (KO) HeLa cells, using [KO Validated] MAPKAPK3 Rabbit polyclonal antibody (STJ11100068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89138/357587/STJ11100068_1__03762.1713121722.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MAPKAPK3 knockout (KO) HeLa cells, using [KO Validated] MAPKAPK3 Rabbit polyclonal antibody (STJ11100068) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
