| Post Translational Modifications | Phosphorylated by MAP3K3. Phosphorylation at Ser-247 promotes its association with CCAR2. Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1. |
| Function | Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition. |
| Protein Name | Proteasome Activator Complex Subunit 311s Regulator Complex Subunit GammaReg-GammaActivator Of Multicatalytic Protease Subunit 3Ki Nuclear AutoantigenProteasome Activator 28 Subunit GammaPa28gPa28gamma |
| Database Links | Reactome: R-HSA-9907900 |
| Cellular Localisation | NucleusCytoplasmLocalizes To The Cytoplasm During Mitosis Following Nuclear Envelope Breakdown At This Distinct Stage Of The Cell Cycle Which Allows Its Interaction With Map3k3 Kinase |
| Alternative Antibody Names | Anti-Proteasome Activator Complex Subunit 3 antibodyAnti-11s Regulator Complex Subunit Gamma antibodyAnti-Reg-Gamma antibodyAnti-Activator Of Multicatalytic Protease Subunit 3 antibodyAnti-Ki Nuclear Autoantigen antibodyAnti-Proteasome Activator 28 Subunit Gamma antibodyAnti-Pa28g antibodyAnti-Pa28gamma antibodyAnti-PSME3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/98779/382828/STJ119994_1__38128.1713151329.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/98779/382828/STJ119994_1__38128.1713151329.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSME3 knockout (KO) 293T cells, using [KO Validated] PSME3 Rabbit polyclonal antibody (STJ119994) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
