• Western blot analysis of extracts of various cell lines, using PSAT1 antibody (STJ116059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PSAT1 antibody (1-370) (STJ116059)

SKU:
STJ116059

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSAT1
Gene ID: 29968
Uniprot ID: SERC_HUMAN
Immunogen Region: 1-370
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human PSAT1 (NP_478059.1).
Immunogen Sequence: MDAPRQVVNFGPGPAKLPHS VLLEIQKELLDYKGVGISVL EMSHRSSDFAKIINNTENLV RELLAVPDNYKVIFLQGGGC GQFSAVPLNLIGLKAGRCAD YVVTGAWSAKAAEEAKKFGT INIVHPKLGSYTKIPDPSTW NLNPDASYVYYCANETVHGV EFDFIPDVKGAVLVCDMSSN FLSKPVDVSKFGVIFAGAQK NVGSAGVTVVIVRDDLLGFA LRECPSVLEYKVQAGNSSL
Tissue Specificity Expressed at high levels in the brain, liver, kidney and pancreas, and very weakly expressed in the thymus, prostate, testis and colon.
Function Involved in L-serine biosynthesis via the phosphorylated pathway, a three-step pathway converting the glycolytic intermediate 3-phospho-D-glycerate into L-serine. Catalyzes the second step, that is the pyridoxal 5'-phosphate-dependent transamination of 3-phosphohydroxypyruvate and L-glutamate to O-phosphoserine (OPS) and alpha-ketoglutarate.
Protein Name Phosphoserine Aminotransferase
Phosphohydroxythreonine Aminotransferase
Psat
Database Links Reactome: R-HSA-977347
Alternative Antibody Names Anti-Phosphoserine Aminotransferase antibody
Anti-Phosphohydroxythreonine Aminotransferase antibody
Anti-Psat antibody
Anti-PSAT1 antibody
Anti-PSA antibody

Information sourced from Uniprot.org

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