• Western blot analysis of extracts of various cell lines, using PRPF8 Rabbit mAb (STJ11102107) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.
  • Immunohistochemistry of paraffin-embedded rat brain using PRPF8 Rabbit mAb (STJ11102107) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human colon using PRPF8 Rabbit mAb (STJ11102107) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using PRPF8 Rabbit mAb (STJ11102107) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of U-2 OS cells using PRPF8 Rabbit mAb (STJ11102107) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRPF8-antibody [ARC1038] (STJ11102107)


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Host: Rabbit
Applications: WB, IHC, IF
Reactivity: Human, Mouse, Rat
Short Description: Rabbit monoclonal antibody anti-PRPF8 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.
Clonality: Monoclonal
Clone ID: ARC1038
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Gene Symbol: PRPF8
Gene ID: 10594
Uniprot ID: PRP8_HUMAN
Immunogen: A synthesized peptide derived from human PRPF8
Tissue Specificity Widely expressed.
Function Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
Protein Name Pre-Mrna-Processing-Splicing Factor 8
220 Kda U5 Snrnp-Specific Protein
Prp8 Homolog
Splicing Factor Prp8
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Pre-Mrna-Processing-Splicing Factor 8 antibody
Anti-220 Kda U5 Snrnp-Specific Protein antibody
Anti-Prp8 Homolog antibody
Anti-Splicing Factor Prp8 antibody
Anti-P220 antibody
Anti-PRPF8 antibody
Anti-PRPC8 antibody

Information sourced from Uniprot.org

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