Immunofluorescence analysis of U-2 OS cells using PRPF8 Rabbit monoclonal antibody (STJ11102107) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRPF8 antibody (2200-2335) [S7MR] (STJ11102107)

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STJ11102107
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRPF8
Gene ID: 10594
Uniprot ID: PRP8_HUMAN
Immunogen Region: 2200-2335
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 2200-2335 of human PRPF8 (Q6P2Q9).
Immunogen Sequence: MADNPSWDGEKTIIITCSFT PGSCTLTAYKLTPSGYEWGR QNTDKGNNPKGYLPSHYERV QMLLSDRFLGFFMVPAQSSW NYNFMGVRHDPNMKYELQLA NPKEFYHEVHRPSHFLNFAL LQEGEVYSADREDLYA
Tissue Specificity Widely expressed.
Function Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
Protein Name Pre-Mrna-Processing-Splicing Factor 8
220 Kda U5 Snrnp-Specific Protein
Prp8 Homolog
Splicing Factor Prp8
P220
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-72165
Cellular Localisation Nucleus
Nucleus Speckle
Alternative Antibody Names Anti-Pre-Mrna-Processing-Splicing Factor 8 antibody
Anti-220 Kda U5 Snrnp-Specific Protein antibody
Anti-Prp8 Homolog antibody
Anti-Splicing Factor Prp8 antibody
Anti-P220 antibody
Anti-PRPF8 antibody
Anti-PRPC8 antibody

Information sourced from Uniprot.org

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