| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PRPF6 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 110kDa band observed in nuclear lysates of cell line Jurkat (calculated MW of 107kDa according to NP_036601.2). Recommended concentration: 1-3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PRPF6 |
| Gene ID: | 24148 |
| Uniprot ID: | PRP6_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_036601.2 |
| Immunogen Sequence: | TGENHTSVDPRQTQ |
| Post Translational Modifications | Phosphorylated by PRP4K during spliceosome assembly. |
| Function | Involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation. |
| Protein Name | Pre-Mrna-Processing Factor 6Androgen Receptor N-Terminal Domain-Transactivating Protein 1Ant-1Prp6 HomologU5 Snrnp-Associated 102 Kda ProteinU5-102 Kda Protein |
| Database Links | Reactome: R-HSA-72163Reactome: R-HSA-72165 |
| Cellular Localisation | NucleusNucleoplasmNucleus SpeckleLocalized In Splicing Speckles |
| Alternative Antibody Names | Anti-Pre-Mrna-Processing Factor 6 antibodyAnti-Androgen Receptor N-Terminal Domain-Transactivating Protein 1 antibodyAnti-Ant-1 antibodyAnti-Prp6 Homolog antibodyAnti-U5 Snrnp-Associated 102 Kda Protein antibodyAnti-U5-102 Kda Protein antibodyAnti-PRPF6 antibodyAnti-C20orf14 antibody |
Information sourced from Uniprot.org
