Western blot detection of Prominin-1 expression in CHO-k1 cells non-transfected (A) or transfected (B) with Prominin-1 and using Prominin-1 mouse mAb (1:1000 diluted).Predicted band size:97KDa.Observed band size:133KDa.

Anti-PROM1 antibody [6H10-F1-C11] (STJ99199)

SKU:
STJ99199
£144.00 - £348.00
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Host: Mouse
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Mouse monoclonal antibody anti-Prominin-1 is suitable for use in Western Blot research applications.
Clonality : Monoclonal
Clone ID : 6H10-F1-C11
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PROM1
Gene ID: 8842
Uniprot ID: PROM1_HUMAN
Specificity: This antibody detects endogenous levels of Prominin-1 and does not cross-react with related proteins.
Immunogen: Purified recombinant human Prominin-1 protein fragments expressed in E.coli.
Post Translational Modifications Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Function May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
Protein Name Prominin-1
Antigen Ac133
Prominin-Like Protein 1
Cd Antigen Cd133
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Microvillus Membrane
Cilium
Photoreceptor Outer Segment
Endoplasmic Reticulum
Endoplasmic Reticulum-Golgi Intermediate Compartment
Found In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal Fluid
Saliva
Seminal Fluid And Urine
Alternative Antibody Names Anti-Prominin-1 antibody
Anti-Antigen Ac133 antibody
Anti-Prominin-Like Protein 1 antibody
Anti-Cd Antigen Cd133 antibody
Anti-PROM1 antibody
Anti-PROML1 antibody
Anti-MSTP061 antibody

Information sourced from Uniprot.org

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