| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRNP |
| Gene ID: | 5621 |
| Uniprot ID: | PRIO_HUMAN |
| Immunogen Region: | 23-230 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human Prion Protein (NP_001073592.1). |
| Immunogen Sequence: | KKRPKPGGWNTGGSRYPGQG SPGGNRYPPQGGGGWGQPHG GGWGQPHGGGWGQPHGGGWG QPHGGGWGQGGGTHSQWNKP SKPKTNMKHMAGAAAAGAVV GGLGGYMLGSAMSRPIIHFG SDYEDRYYRENMHRYPNQVY YRPMDEYSNQNNFVHDCVNI TIKQHTVTTTTKGENFTETD VKMMERVVEQMCITQYERES QAYYQRGS |
| Post Translational Modifications | The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. |
| Function | Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. |
| Protein Name | Major Prion Protein Prp Ascr Prp27-30 Prp33-35c Cd Antigen Cd230 |
| Database Links | Reactome: R-HSA-419037 Reactome: R-HSA-9609523 |
| Cellular Localisation | Cell Membrane Lipid-Anchor Gpi-Anchor Golgi Apparatus Targeted To Lipid Rafts Via Association With The Heparan Sulfate Chains Of Gpc1 Colocates In The Presence Of Cu(2+) To Vesicles In Para- And Perinuclear Regions Where Both Proteins Undergo Internalization Heparin Displaces Prnp From Lipid Rafts And Promotes Endocytosis |
| Alternative Antibody Names | Anti-Major Prion Protein antibody Anti-Prp antibody Anti-Ascr antibody Anti-Prp27-30 antibody Anti-Prp33-35c antibody Anti-Cd Antigen Cd230 antibody Anti-PRNP antibody Anti-ALTPRP antibody Anti-PRIP antibody Anti-PRP antibody |
Information sourced from Uniprot.org