| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Major Prion Protein (23-230) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRNP |
| Gene ID: |
5621 |
| Uniprot ID: |
PRIO_HUMAN |
| Immunogen Region: |
23-230 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human Prion Protein (NP_001073592.1). |
| Immunogen Sequence: |
KKRPKPGGWNTGGSRYPGQG SPGGNRYPPQGGGGWGQPHG GGWGQPHGGGWGQPHGGGWG QPHGGGWGQGGGTHSQWNKP SKPKTNMKHMAGAAAAGAVV GGLGGYMLGSAMSRPIIHFG SDYEDRYYRENMHRYPNQVY YRPMDEYSNQNNFVHDCVNI TIKQHTVTTTTKGENFTETD VKMMERVVEQMCITQYERES QAYYQRGS |
| Post Translational Modifications | The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion. |
| Function | Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains. |
| Protein Name | Major Prion ProteinPrpAscrPrp27-30Prp33-35cCd Antigen Cd230 |
| Database Links | Reactome: R-HSA-419037Reactome: R-HSA-9609523 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorGolgi ApparatusTargeted To Lipid Rafts Via Association With The Heparan Sulfate Chains Of Gpc1ColocatesIn The Presence Of Cu(2+)To Vesicles In Para- And Perinuclear RegionsWhere Both Proteins Undergo InternalizationHeparin Displaces Prnp From Lipid Rafts And Promotes Endocytosis |
| Alternative Antibody Names | Anti-Major Prion Protein antibodyAnti-Prp antibodyAnti-Ascr antibodyAnti-Prp27-30 antibodyAnti-Prp33-35c antibodyAnti-Cd Antigen Cd230 antibodyAnti-PRNP antibodyAnti-ALTPRP antibodyAnti-PRIP antibodyAnti-PRP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89103/357560/STJ11100031_1__14868.1713121685.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
