| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PRMT3 (468-421) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 70kDa band observed in lysates of cell line MCF7 (calculated MW of 59.9kDa according to NP_005779.1). The observed molecular weight corresponds to earlier findings in literature |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PRMT3 |
| Gene ID: | 10196 |
| Uniprot ID: | ANM3_HUMAN |
| Immunogen Region: | 468-421 |
| Accession Number: | NP_005779.1; NP_001138638.1; NP_001138639.1 |
| Specificity: | This antibody is expected to recognize all reported isoforms (NP_005779.1; NP_001138638.1; NP_001138639.1). |
| Immunogen Sequence: | DPKTLISEPCGIKH |
| Protein Name | Protein Arginine N-Methyltransferase 3Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3 |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8876725 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Protein Arginine N-Methyltransferase 3 antibodyAnti-Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3 antibodyAnti-PRMT3 antibodyAnti-HRMT1L3 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/94075/375453/STJ115035_1__47849.1713142217.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
