• Western blot analysis of extracts from normal (control) and PRKAR1A knockout (KO) 293T cells, using PRKAR1A antibody (STJ25122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Western blot analysis of extracts from normal (control) and PRKAR1A knockout (KO) 293T cells, using PRKAR1A antibody (STJ25122) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using PRKAR1A antibody (STJ25122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PRKAR1A antibody (1-250) (STJ25122)

SKU:
STJ25122

£129.50 - £642.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAR1A
Gene ID: 5573
Uniprot ID: KAP0_HUMAN
Immunogen Region: 1-250
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PRKAR1A (NP_002725.1).
Immunogen Sequence: MESGSTAASEEARSLRECEL YVQKHNIQALLKDSIVQLCT ARPERPMAFLREYFERLEKE EAKQIQNLQKAGTRTDSRED EISPPPPNPVVKGRRRRGAI SAEVYTEEDAASYVRKVIPK DYKTMAALAKAIEKNVLFSH LDDNERSDIFDAMFSVSFIA GETVIQQGDEGDNFYVIDQG ETDVYVNNEWATSVGEGGSF GELALIYGTPRAATVKAKTN VKLWGIDRDSYRRILMGST
Tissue Specificity Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Post Translational Modifications The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.
Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.
Protein Name Camp-Dependent Protein Kinase Type I-Alpha Regulatory Subunit
Tissue-Specific Extinguisher 1
Tse1
Database Links Reactome: R-HSA-163615
Reactome: R-HSA-164378
Reactome: R-HSA-180024
Reactome: R-HSA-381676
Reactome: R-HSA-432040
Reactome: R-HSA-442720
Reactome: R-HSA-5610787
Reactome: R-HSA-9634597
Reactome: R-HSA-9660821
Reactome: R-HSA-9664323
Reactome: R-HSA-9700645
Reactome: R-HSA-9725370
Reactome: R-HSA-983231
Cellular Localisation Cell Membrane
Alternative Antibody Names Anti-Camp-Dependent Protein Kinase Type I-Alpha Regulatory Subunit antibody
Anti-Tissue-Specific Extinguisher 1 antibody
Anti-Tse1 antibody
Anti-PRKAR1A antibody
Anti-PKR1 antibody
Anti-PRKAR1 antibody
Anti-TSE1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance