Anti-PRKACB antibody (250-351) (STJ27277)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRKACB (250-351) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRKACB |
| Gene ID | 5567 |
| Uniprot ID | KAPCB_HUMAN |
| Immunogen Region | 250-351 |
| Immunogen Sequence | KIVSGKVRFPSHFSSDLKDL LRNLLQVDLTKRFGNLKNGV SDIKTHKWFATTDWIAIYQR KVEAPFIPKFRGSGDTSNFD DYEEEDIRVSITEKCAKEFG EF |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 250-351 of human PKA C-beta (PRKACB) (NP_002722.1). |
Additional Info
| Tissue Specificity | Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. |
| Post Translational Modifications | Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. |
| Function | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR. |
| Protein Name | Camp-Dependent Protein Kinase Catalytic Subunit BetaPka C-Beta |
| Database Links | Reactome: R-HSA-111931Reactome: R-HSA-163358Reactome: R-HSA-163560Reactome: R-HSA-163615Reactome: R-HSA-164378Reactome: R-HSA-180024Reactome: R-HSA-381676Reactome: R-HSA-392517Reactome: R-HSA-422356Reactome: R-HSA-432040Reactome: R-HSA-4420097Reactome: R-HSA-442720Reactome: R-HSA-5610780Reactome: R-HSA-5610783Reactome: R-HSA-5610785Reactome: R-HSA-5610787Reactome: R-HSA-5621575Reactome: R-HSA-5687128Reactome: R-HSA-8853659Reactome: R-HSA-8963896Reactome: R-HSA-9010642Reactome: R-HSA-9634597Reactome: R-HSA-9634600Reactome: R-HSA-9660821Reactome: R-HSA-9664323Reactome: R-HSA-983231Reactome: R-HSA-9856530 |
| Cellular Localisation | CytoplasmCell MembraneMembraneLipid-AnchorNucleusTranslocates Into The Nucleus (Monomeric Catalytic Subunit)The Inactive Holoenzyme Is Found In The Cytoplasm |
| Alternative Antibody Names | Anti-Camp-Dependent Protein Kinase Catalytic Subunit Beta antibodyAnti-Pka C-Beta antibodyAnti-PRKACB antibody |
Information sourced from Uniprot.org