| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRKACB |
| Gene ID: | 5567 |
| Uniprot ID: | KAPCB_HUMAN |
| Immunogen Region: | 250-351 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 250-351 of human PKA C-beta (PRKACB) (NP_002722.1). |
| Immunogen Sequence: | KIVSGKVRFPSHFSSDLKDL LRNLLQVDLTKRFGNLKNGV SDIKTHKWFATTDWIAIYQR KVEAPFIPKFRGSGDTSNFD DYEEEDIRVSITEKCAKEFG EF |
| Tissue Specificity | Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. |
| Post Translational Modifications | Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively. |
| Function | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR. |
| Protein Name | Camp-Dependent Protein Kinase Catalytic Subunit Beta Pka C-Beta |
| Database Links | Reactome: R-HSA-111931 Reactome: R-HSA-163358 Reactome: R-HSA-163560 Reactome: R-HSA-163615 Reactome: R-HSA-164378 Reactome: R-HSA-180024 Reactome: R-HSA-381676 Reactome: R-HSA-392517 Reactome: R-HSA-422356 Reactome: R-HSA-432040 Reactome: R-HSA-4420097 Reactome: R-HSA-442720 Reactome: R-HSA-5610780 Reactome: R-HSA-5610783 Reactome: R-HSA-5610785 Reactome: R-HSA-5610787 Reactome: R-HSA-5621575 Reactome: R-HSA-5687128 Reactome: R-HSA-8853659 Reactome: R-HSA-8963896 Reactome: R-HSA-9010642 Reactome: R-HSA-9634597 Reactome: R-HSA-9634600 Reactome: R-HSA-9660821 Reactome: R-HSA-9664323 Reactome: R-HSA-983231 |
| Cellular Localisation | Cytoplasm Cell Membrane Membrane Lipid-Anchor Nucleus Translocates Into The Nucleus (Monomeric Catalytic Subunit) The Inactive Holoenzyme Is Found In The Cytoplasm |
| Alternative Antibody Names | Anti-Camp-Dependent Protein Kinase Catalytic Subunit Beta antibody Anti-Pka C-Beta antibody Anti-PRKACB antibody |
Information sourced from Uniprot.org