| Host: | Goat |
| Applications: | Pep-ELISA/IF/IHC/FC |
| Reactivity: | Human/Mouse/Rat/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PRKAB2 (Internal) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000. IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 3.75µg/ml. IF: Strong expression of the protein seen in the nuclei of MCF7 and A431 cells. Recommended |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PRKAB2 |
| Gene ID: | 5565 |
| Uniprot ID: | AAKB2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_005390.1 |
| Immunogen Sequence: | PYGQEMYAFRSE |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Beta-2 Ampk Subunit Beta-2 |
| Database Links | Reactome: R-HSA-1445148 Reactome: R-HSA-1632852 Reactome: R-HSA-163680 Reactome: R-HSA-200425 Reactome: R-HSA-2151209 Reactome: R-HSA-380972 Reactome: R-HSA-5628897 Reactome: R-HSA-6804756 Reactome: R-HSA-9613354 Reactome: R-HSA-9619483 |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Beta-2 antibody Anti-Ampk Subunit Beta-2 antibody Anti-PRKAB2 antibody |
Information sourced from Uniprot.org