Western blot analysis of extracts of Mouse liver, using PRDM14 antibody (STJ28585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-PRDM14 antibody (STJ28585)

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STJ28585
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Prdm14
Gene ID: 383491
Uniprot ID: PRD14_MOUSE
Specificity: Recombinant protein of mouse PRDM14
Tissue Specificity Restricted to embryonic stem cells and primordial germ cells. Not detected in epiblast-derived stem cells.
Function Transcription factor that has both positive and negative roles on transcription. Plays a role in cellular pluripotency. Essential for germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation.
Protein Name Pr Domain Zinc Finger Protein 14
Pr Domain-Containing Protein 14
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pr Domain Zinc Finger Protein 14 antibody
Anti-Pr Domain-Containing Protein 14 antibody
Anti-Prdm14 antibody

Information sourced from Uniprot.org

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