• Immunohistochemistry analysis of paraffin-embedded mouse liver using PP2A-B55 Alpha/PR55 Alpha/PPP2R2A antibody (STJ25087) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using PP2A-B55 Alpha/PR55 Alpha/PPP2R2A antibody (STJ25087) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using PP2A-B55 Alpha/PR55 Alpha/PPP2R2A antibody (STJ25087) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PP2A-B55 Alpha/PR55 Alpha/PPP2R2A antibody (STJ25087) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PPP2R2A antibody (1-447) (STJ25087)

SKU:
STJ25087

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP2R2A (1-447) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP2R2A
Gene ID: 5520
Uniprot ID: 2ABA_HUMAN
Immunogen Region: 1-447
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-447 of human PP2A-B55 Alpha/PR55 Alpha/PPP2R2A (NP_002708.1).
Immunogen Sequence: MAGAGGGNDIQWCFSQVKGA VDDDVAEADIISTVEFNHSG ELLATGDKGGRVVIFQQEQE NKIQSHSRGEYNVYSTFQSH EPEFDYLKSLEIEEKINKIR WLPQKNAAQFLLSTNDKTIK LWKISERDKRPEGYNLKEED GRYRDPTTVTTLRVPVFRPM DLMVEASPRRIFANAHTYHI NSISINSDYETYLSADDLRI NLWHLEITDRSFNIVDIKPA NMEELTEVITAAEFHPNSC
Tissue Specificity Expressed in all tissues examined.
Function The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Essential for serine/threonine-protein phosphatase 2A-mediated dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint.
Protein Name Serine/Threonine-Protein Phosphatase 2a 55 Kda Regulatory Subunit B Alpha Isoform
Pp2a Subunit B Isoform B55-Alpha
Pp2a Subunit B Isoform Pr55-Alpha
Pp2a Subunit B Isoform R2-Alpha
Pp2a Subunit B Isoform Alpha
Database Links Reactome: R-HSA-2995383
Reactome: R-HSA-69231
Reactome: R-HSA-69273
Reactome: R-HSA-975957
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a 55 Kda Regulatory Subunit B Alpha Isoform antibody
Anti-Pp2a Subunit B Isoform B55-Alpha antibody
Anti-Pp2a Subunit B Isoform Pr55-Alpha antibody
Anti-Pp2a Subunit B Isoform R2-Alpha antibody
Anti-Pp2a Subunit B Isoform Alpha antibody
Anti-PPP2R2A antibody

Information sourced from Uniprot.org

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