| Host: |
Rabbit |
| Applications: |
WB/IHC-F/IHC-P/ICC/IF/IP |
| Reactivity: |
Human/Mouse/Rat/Hamster |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-PP2A alpha/beta (277-294 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
R04-4G4 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: |
Affinity Purified |
| Concentration: |
0.3 mg/ml |
| Dilution Range: |
WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:20 |
| Storage Instruction: |
Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: |
PPP2CA |
| Gene ID: |
5515 |
| Uniprot ID: |
PP2AA_HUMAN |
| Immunogen Region: |
277-294 aa |
| Immunogen: |
A synthetic peptide of human PP2A alpha |
| Post Translational Modifications | Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. Polyubiquitinated, leading to its degradation by the proteasome. |
| Function | Catalytic subunit of protein phosphatase 2A (PP2A), a serine/threonine phosphatase involved in the regulation of a wide variety of enzymes, signal transduction pathways, and cellular events. PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGO2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I. Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'. Mediates dephosphorylation of WEE1, preventing its ubiquitin-mediated proteolysis, increasing WEE1 protein levels, and promoting the G2/M checkpoint. Mediates dephosphorylation of MYC.promoting its ubiquitin-mediated proteolysis: interaction with AMBRA1 enhances interaction between PPP2CA and MYC. Mediates dephosphorylation of FOXO3.promoting its stabilization: interaction with AMBRA1 enhances interaction between PPP2CA and FOXO3. Catalyzes dephosphorylation of the pyrin domain of NLRP3, promoting assembly of the NLRP3 inflammasome. Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation. |
| Protein Name | Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha IsoformPp2a-AlphaReplication Protein CRp-C |
| Database Links | Reactome: R-HSA-113501Reactome: R-HSA-1295596Reactome: R-HSA-141444Reactome: R-HSA-163685Reactome: R-HSA-163767Reactome: R-HSA-180024Reactome: R-HSA-195253Reactome: R-HSA-196299Reactome: R-HSA-198753Reactome: R-HSA-202670Reactome: R-HSA-2465910Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2995383Reactome: R-HSA-389513Reactome: R-HSA-432142Reactome: R-HSA-4641262Reactome: R-HSA-5339716Reactome: R-HSA-5358747Reactome: R-HSA-5358749Reactome: R-HSA-5358751Reactome: R-HSA-5358752Reactome: R-HSA-5467337Reactome: R-HSA-5467340Reactome: R-HSA-5467348Reactome: R-HSA-5663220Reactome: R-HSA-5673000Reactome: R-HSA-5675221Reactome: R-HSA-6804757Reactome: R-HSA-6811558Reactome: R-HSA-68877Reactome: R-HSA-69231Reactome: R-HSA-69273Reactome: R-HSA-9634600Reactome: R-HSA-9648025Reactome: R-HSA-975957Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmNucleusChromosomeCentromereCytoskeletonSpindle PoleIn Prometaphase CellsBut Not In Anaphase CellsLocalizes At CentromeresDuring MitosisAlso Found At Spindle PolesCentromeric Localization Requires The Presence Of Sgo2 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Phosphatase 2a Catalytic Subunit Alpha Isoform antibodyAnti-Pp2a-Alpha antibodyAnti-Replication Protein C antibodyAnti-Rp-C antibodyAnti-PPP2CA antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
