| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Protein phosphatase 1 regulatory subunit 15A (430-490 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:10000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PPP1R15A |
| Gene ID: |
23645 |
| Uniprot ID: |
PR15A_HUMAN |
| Immunogen Region: |
430-490 aa |
| Specificity: |
The antibody detects endogenous GADD34 |
| Immunogen: |
Synthetic peptide from the human protein at the amino acid range 430-490 |
| Function | Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Acts also a viral restriction factor by attenuating HIV-1 replication. Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 15aGrowth Arrest And Dna Damage-Inducible Protein Gadd34Myeloid Differentiation Primary Response Protein Myd116 Homolog |
| Database Links | Reactome: R-HSA-2173788Reactome: R-HSA-9648895 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideMitochondrion Outer MembraneAssociates With Membranes Via An N-Terminal Amphipathic Intramembrane Region |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 15a antibodyAnti-Growth Arrest And Dna Damage-Inducible Protein Gadd34 antibodyAnti-Myeloid Differentiation Primary Response Protein Myd116 Homolog antibodyAnti-PPP1R15A antibodyAnti-GADD34 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
