Anti-PPP1R15A antibody (375-674) (STJ11101176)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PPP1R15A (375-674) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PPP1R15A |
| Gene ID | 23645 |
| Uniprot ID | PR15A_HUMAN |
| Immunogen Region | 375-674 |
| Immunogen Sequence | ASSSTPATGVFLKSWVYQPG EDTEEEEDEDSDTGSAEDER EAETSASTPPASAFLKAWVY RPGEDTEEEEDEDVDSEDKE DDSEAALGEAESDPHPSHPD QRAHFRGWGYRPGKETEEEE AAEDWGEAEPCPFRVAIYVP GEKPPPPWAPPRLPLRLQRR LKRPETPTHDPDPETPLKAR KVRFSEKVTVHFLAVWAGPA QAARQGPWEQLARDRSRFAR RITQAQEELSPCLTPAARA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 375-674 of human PPP1R15A (NP_055145.3). |
Additional Info
| Post Translational Modifications | Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN.which impairs its antiproliferative activity. Phosphorylation at Tyr-262 enhances proteasomal degradation, this position is dephosphorylated by PTPN2. Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association. |
| Function | Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Also acts a viral restriction factor by attenuating HIV-1 replication. Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 15aGrowth Arrest And Dna Damage-Inducible Protein Gadd34Myeloid Differentiation Primary Response Protein Myd116 Homolog |
| Database Links | Reactome: R-HSA-2173788Reactome: R-HSA-9648895 |
| Cellular Localisation | Endoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideMitochondrion Outer MembraneAssociates With Membranes Via An N-Terminal Amphipathic Intramembrane Region |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 15a antibodyAnti-Growth Arrest And Dna Damage-Inducible Protein Gadd34 antibodyAnti-Myeloid Differentiation Primary Response Protein Myd116 Homolog antibodyAnti-PPP1R15A antibodyAnti-GADD34 antibody |
Information sourced from Uniprot.org