| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPP1R15A |
| Gene ID: | 23645 |
| Uniprot ID: | PR15A_HUMAN |
| Immunogen Region: | 375-674 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 375-674 of human PPP1R15A (NP_055145.3). |
| Immunogen Sequence: | ASSSTPATGVFLKSWVYQPG EDTEEEEDEDSDTGSAEDER EAETSASTPPASAFLKAWVY RPGEDTEEEEDEDVDSEDKE DDSEAALGEAESDPHPSHPD QRAHFRGWGYRPGKETEEEE AAEDWGEAEPCPFRVAIYVP GEKPPPPWAPPRLPLRLQRR LKRPETPTHDPDPETPLKAR KVRFSEKVTVHFLAVWAGPA QAARQGPWEQLARDRSRFAR RITQAQEELSPCLTPAARA |
| Post Translational Modifications | Phosphorylated at multiple Ser/Thr residues. Phosphorylated on tyrosine by LYN.which impairs its antiproliferative activity. Phosphorylation at Tyr-262 enhances proteasomal degradation, this position is dephosphorylated by PTPN2. Polyubiquitinated. Exhibits a rapid proteasomal degradation with a half-life under 1 hour, ubiquitination depends on endoplasmic reticulum association. |
| Function | Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Acts also a viral restriction factor by attenuating HIV-1 replication. Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 15a Growth Arrest And Dna Damage-Inducible Protein Gadd34 Myeloid Differentiation Primary Response Protein Myd116 Homolog |
| Database Links | Reactome: R-HSA-2173788 Reactome: R-HSA-9648895 |
| Cellular Localisation | Endoplasmic Reticulum Membrane Peripheral Membrane Protein Cytoplasmic Side Mitochondrion Outer Membrane Associates With Membranes Via An N-Terminal Amphipathic Intramembrane Region |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 15a antibody Anti-Growth Arrest And Dna Damage-Inducible Protein Gadd34 antibody Anti-Myeloid Differentiation Primary Response Protein Myd116 Homolog antibody Anti-PPP1R15A antibody Anti-GADD34 antibody |
Information sourced from Uniprot.org