| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PPP1R14A (1-147) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PPP1R14A |
| Gene ID: |
94274 |
| Uniprot ID: |
PP14A_HUMAN |
| Immunogen Region: |
1-147 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1). |
| Immunogen Sequence: |
MAAQRLGKRVLSKLQSPSRA RGPGGSPGGLQKRHARVTVK YDRRELQRRLDVEKWIDGRL EELYRGMEADMPDEINIDEL LELESEEERSRKIQGLLKSC GKPVEDFIQELLAKLQGLHR QPGLRQPSPSHDGSLSPLQD RARTAHP |
| Tissue Specificity | Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. |
| Function | Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. |
| Protein Name | Protein Phosphatase 1 Regulatory Subunit 14a17 Kda Pkc-Potentiated Inhibitory Protein Of Pp1Protein Kinase C-Potentiated Inhibitor Protein Of 17 KdaCpi-17 |
| Database Links | Reactome: R-HSA-5625740 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Protein Phosphatase 1 Regulatory Subunit 14a antibodyAnti-17 Kda Pkc-Potentiated Inhibitory Protein Of Pp1 antibodyAnti-Protein Kinase C-Potentiated Inhibitor Protein Of 17 Kda antibodyAnti-Cpi-17 antibodyAnti-PPP1R14A antibodyAnti-CPI17 antibodyAnti-PPP1INL antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/101832/390042/STJ28552_1__58226.1713159925.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and POLE3 knockout (KO) 293T cells, using [KO Validated] POLE3 Rabbit polyclonal antibody (STJ28552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
