• Western blot analysis of lysates from NIH/3T3 cells, using MYPT1 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of lysates from HeLa, 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) loading contrl antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MYPT1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PPP1R12A antibody (621-670) (STJ94320)

SKU:
STJ94320

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein phosphatase 1 regulatory subunit 12A (621-670) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP1R12A
Gene ID: 4659
Uniprot ID: MYPT1_HUMAN
Immunogen Region: 621-670
Specificity: MYPT1 Polyclonal Antibody detects endogenous levels of MYPT1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from human MYPT1. AA range:621-670
Tissue Specificity Expressed in striated muscles, specifically in type 2a fibers (at protein level).
Post Translational Modifications Phosphorylated by CIT (Rho-associated kinase). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR. In vitro, phosphorylation of Ser-695 by PKA and PKG appears to prevent phosphorylation of the inhibitory site Thr-696, probably mediated by PRKG1. Phosphorylation at Ser-445, Ser-472 and Ser-910 by NUAK1 promotes interaction with 14-3-3, leading to inhibit interaction with myosin light chain MLC2, preventing dephosphorylation of MLC2. May be phosphorylated at Thr-696 by DMPK.may inhibit the myosin phosphatase activity. Phosphorylated at Ser-473 by CDK1 during mitosis, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates PPP1R12A.
Function Key regulator of protein phosphatase 1C (PPP1C). Mediates binding to myosin. As part of the PPP1C complex, involved in dephosphorylation of PLK1. Capable of inhibiting HIF1AN-dependent suppression of HIF1A activity.
Protein Name Protein Phosphatase 1 Regulatory Subunit 12a
Myosin Phosphatase-Targeting Subunit 1
Myosin Phosphatase Target Subunit 1
Protein Phosphatase Myosin-Binding Subunit
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Cellular Localisation Cytoplasm
Cytoskeleton
Stress Fiber
Also Along Actomyosin Filaments
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 12a antibody
Anti-Myosin Phosphatase-Targeting Subunit 1 antibody
Anti-Myosin Phosphatase Target Subunit 1 antibody
Anti-Protein Phosphatase Myosin-Binding Subunit antibody
Anti-PPP1R12A antibody
Anti-MBS antibody
Anti-MYPT1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance