| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPM1F |
| Gene ID: | 9647 |
| Uniprot ID: | PPM1F_HUMAN |
| Immunogen Region: | 1-190 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human PPM1F (NP_055449.1). |
| Immunogen Sequence: | MSSGAPQKSSPMASGAEETP GFLDTLLQDFPALLNPEDPL PWKAPGTVLSQEEVEGELAE LAMGFLGSRKAPPPLAAALA HEAVSQLLQTDLSEFRKLPR EEEEEEEDDDEEEKAPVTLL DAQSLAQSFFNRLWEVAGQW QKQVPLAARASQRQWLVSIH AIRNTRRKMEDRHVSLPSFN QLFGLSDPVN |
| Function | Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis. |
| Protein Name | Protein Phosphatase 1fCa(2+/Calmodulin-Dependent Protein Kinase PhosphataseCam-Kinase PhosphataseCamkpasePartner Of Pix 2Protein Fem-2 HomologHfem-2 |
| Database Links | Reactome: R-HSA-9617324 |
| Alternative Antibody Names | Anti-Protein Phosphatase 1f antibodyAnti-Ca(2+/Calmodulin-Dependent Protein Kinase Phosphatase antibodyAnti-Cam-Kinase Phosphatase antibodyAnti-Camkpase antibodyAnti-Partner Of Pix 2 antibodyAnti-Protein Fem-2 Homolog antibodyAnti-Hfem-2 antibodyAnti-PPM1F antibodyAnti-KIAA0015 antibodyAnti-POPX2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/96581/379140/STJ117622_1__97810.1713146869.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and MYOF knockout (KO) HeLa (KO) cells, using [KO Validated] MYOF Rabbit polyclonal antibody (STJ117622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
