• Immunofluorescence analysis of MCF7 cells using PPL antibody (STJ29030). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PPL antibody (STJ29030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PPL antibody (1-130) (STJ29030)

SKU:
STJ29030

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPL
Gene ID: 5493
Uniprot ID: PEPL_HUMAN
Immunogen Region: 1-130
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PPL (NP_002696.3).
Immunogen Sequence: MNSLFRKRNKGKYSPTVQTR SISNKELSELIEQLQKNADQ VEKNIVDTEAKMQSDLARLQ EGRQPEHRDVTLQKVLDSEK LLYVLEADAAIAKHMKHPQG DMIAEDIRQLKERVTNLRGK HKQIYRLAVK
Tissue Specificity Expressed in stratified squamous epithelia and in some other epithelia.
Function Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.
Protein Name Periplakin
190 Kda Paraneoplastic Pemphigus Antigen
195 Kda Cornified Envelope Precursor Protein
Database Links Reactome: R-HSA-6809371
Reactome: R-HSA-8851680
Cellular Localisation Cell Junction
Desmosome
Cytoplasm
Cytoskeleton
Cell Membrane
Alternative Antibody Names Anti-Periplakin antibody
Anti-190 Kda Paraneoplastic Pemphigus Antigen antibody
Anti-195 Kda Cornified Envelope Precursor Protein antibody
Anti-PPL antibody
Anti-KIAA0568 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance