| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PPIE |
| Gene ID: | 10450 |
| Uniprot ID: | PPIE_HUMAN |
| Immunogen Region: | 1-301 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of human PPIE (NP_006103.1). |
| Immunogen Sequence: | MATTKRVLYVGGLAEEVDDK VLHAAFIPFGDITDIQIPLD YETEKHRGFAFVEFELAEDA AAAIDNMNESELFGRTIRVN LAKPMRIKEGSSRPVWSDDD WLKKFSGKTLEENKEEEGSE PPKAETQEGEPIAKKARSNP QVYMDIKIGNKPAGRIQMLL RSDVVPMTAENFRCLCTHEK GFGFKGSSFHRIIPQFMCQG GDFTNHNGTGGKSIYGKKFD DENFILKHTGPGLLSMANS |
| Tissue Specificity | Found in all the examined tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
| Function | Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity.this requires proline isomerase activity. |
| Protein Name | Peptidyl-Prolyl Cis-Trans Isomerase EPpiase ECyclophilin ECyclophilin-33Rotamase E |
| Database Links | Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-6798695Reactome: R-HSA-72163 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Peptidyl-Prolyl Cis-Trans Isomerase E antibodyAnti-Ppiase E antibodyAnti-Cyclophilin E antibodyAnti-Cyclophilin-33 antibodyAnti-Rotamase E antibodyAnti-PPIE antibodyAnti-CYP33 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and NOSIP knockout (KO) 293T cells using [KO Validated] NOSIP Rabbit polyclonal antibody (STJ11104394) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177038/368258/STJ11104394_1__88977.1713133758.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NOSIP knockout (KO) 293T cells using [KO Validated] NOSIP Rabbit polyclonal antibody (STJ11104394) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
