• Immunofluorescence analysis of U-2 OS cells using POU4F2 Rabbit polyclonal antibody (STJ11103925) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse brain, using POU4F2 Rabbit polyclonal antibody (STJ11103925) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-POU4F2 antibody (1-100) (STJ11103925)

SKU:
STJ11103925

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pou Domain-Class 4-Transcription Factor 2 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU4F2
Gene ID: 5458
Uniprot ID: PO4F2_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human POU4F2 (NP_004566.2).
Immunogen Sequence: MMMMSLNSKQAFSMPHGGSL HVEPKYSALHSTSPGSSAPI APSASSPSSSSNAGGGGGGG GGGGGGGGRSSSSSSSGSSG GGGSEAMRRACLPTPPSNIF
Tissue Specificity Expressed in the brain. Expressed in the ganglion cell layer of the retina.
Function Tissue-specific DNA-binding transcription factor involved in the development and differentiation of target cells. Functions either as activator or repressor modulating the rate of target gene transcription through RNA polymerase II enzyme in a promoter-dependent manner. Binds to the consensus octamer motif 5'-ATA/TAT/ATA/TA-3' of promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. Binds to an octamer site to form a ternary complex with ISL1.cooperates positively with ISL1 and ISL2 to potentiate transcriptional activation of RGC target genes being involved in RGC fate commitment in the developing retina and RGC axon formation and pathfinding. Inhibits DLX1 and DLX2 transcriptional activities preventing DLX1- and DLX2-mediated ability to promote amacrine cell fate specification. In cooperation with TP53 potentiates transcriptional activation of BAX promoter activity increasing neuronal cell apoptosis. Negatively regulates BAX promoter activity in the absence of TP53. Acts as a transcriptional coactivator via its interaction with the transcription factor ESR1 by enhancing its effect on estrogen response element (ERE)-containing promoter. Antagonizes the transcriptional stimulatory activity of POU4F1 by preventing its binding to an octamer motif. Involved in TNFSF11-mediated terminal osteoclast differentiation.
Protein Name Pou Domain - Class 4 - Transcription Factor 2
Brain-Specific Homeobox/Pou Domain Protein 3b
Brain-3b
Brn-3b
Database Links Reactome: R-HSA-6804759
Cellular Localisation Nucleus
Nucleus Speckle
Cytoplasm
Alternative Antibody Names Anti-Pou Domain - Class 4 - Transcription Factor 2 antibody
Anti-Brain-Specific Homeobox/Pou Domain Protein 3b antibody
Anti-Brain-3b antibody
Anti-Brn-3b antibody
Anti-POU4F2 antibody
Anti-BRN3B antibody

Information sourced from Uniprot.org

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