| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Cow/Pig |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-POU3F2/BRN2/OCT7 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000. WB: Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_0329 |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | POU3F2 |
| Gene ID: | 5454 |
| Uniprot ID: | PO3F2_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_005595.2 |
| Immunogen Sequence: | AQSLVQGDYGALQSN |
| Function | Transcription factor that plays a key role in neuronal differentiation. Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides. Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes. |
| Protein Name | Pou Domain - Class 3 - Transcription Factor 2 Brain-Specific Homeobox/Pou Domain Protein 2 Brain-2 Brn-2 Nervous System-Specific Octamer-Binding Transcription Factor N-Oct-3 Octamer-Binding Protein 7 Oct-7 Octamer-Binding Transcription Factor 7 Otf-7 |
| Database Links | Reactome: R-HSA-9619665 Reactome: R-HSA-9824594 Reactome: R-HSA-9856649 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pou Domain - Class 3 - Transcription Factor 2 antibody Anti-Brain-Specific Homeobox/Pou Domain Protein 2 antibody Anti-Brain-2 antibody Anti-Brn-2 antibody Anti-Nervous System-Specific Octamer-Binding Transcription Factor N-Oct-3 antibody Anti-Octamer-Binding Protein 7 antibody Anti-Oct-7 antibody Anti-Octamer-Binding Transcription Factor 7 antibody Anti-Otf-7 antibody Anti-POU3F2 antibody Anti-BRN2 antibody Anti-OCT7 antibody Anti-OTF7 antibody |
Information sourced from Uniprot.org