Anti-POU3F2/BRN2/OCT7 antibody (Internal) (STJ72234)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-POU3F2/BRN2/OCT7 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Cow/Pig |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_0329 |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | POU3F2 |
| Gene ID | 5454 |
| Uniprot ID | PO3F2_HUMAN |
| Accession Number | NP_005595.2 |
| Immunogen Region | Internal |
| Immunogen Sequence | AQSLVQGDYGALQSN |
Additional Info
| Function | Transcription factor that plays a key role in neuronal differentiation. Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides. Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes. |
| Protein Name | Pou Domain - Class 3 - Transcription Factor 2Brain-Specific Homeobox/Pou Domain Protein 2Brain-2Brn-2Nervous System-Specific Octamer-Binding Transcription Factor N-Oct-3Octamer-Binding Protein 7Oct-7Octamer-Binding Transcription Factor 7Otf-7 |
| Database Links | Reactome: R-HSA-9619665Reactome: R-HSA-9824594Reactome: R-HSA-9856649 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pou Domain - Class 3 - Transcription Factor 2 antibodyAnti-Brain-Specific Homeobox/Pou Domain Protein 2 antibodyAnti-Brain-2 antibodyAnti-Brn-2 antibodyAnti-Nervous System-Specific Octamer-Binding Transcription Factor N-Oct-3 antibodyAnti-Octamer-Binding Protein 7 antibodyAnti-Oct-7 antibodyAnti-Octamer-Binding Transcription Factor 7 antibodyAnti-Otf-7 antibodyAnti-POU3F2 antibodyAnti-BRN2 antibodyAnti-OCT7 antibodyAnti-OTF7 antibody |
Information sourced from Uniprot.org