Western blot analysis of lysates from wild type (WT) and 293T cells transfected with POU1F1 using POU1F1 Rabbit polyclonal antibody (STJ119954) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-POU1F1 antibody (124-273) (STJ119954)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ119954
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General Information

Short DescriptionRabbit polyclonal POU1F1 (124-273) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:100-1:500
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPOU1F1
Gene ID5449
Uniprot IDPIT1_HUMAN
Immunogen Region124-273
Immunogen SequenceMDSPEIRELEKFANEFKVRR IKLGYTQTNVGEALAAVHGS EFSQTTICRFENLQLSFKNA CKLKAILSKWLEEAEQVGAL YNEKVGANERKRKRRTTISI AAKDALERHFGEQNKPSSQE IMRMAEELNLEKEVVRVWFC NRRQREKRVK
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 124-273 of human POU1F1 (NP_000297.1?).

Additional Info

Function Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Specifically binds to the consensus sequence 5'-TAAAT-3'. Activates growth hormone and prolactin genes.
Protein Name Pituitary-Specific Positive Transcription Factor 1
Pit-1
Growth Hormone Factor 1
Ghf-1
Database Links
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pituitary-Specific Positive Transcription Factor 1 antibody
Anti-Pit-1 antibody
Anti-Growth Hormone Factor 1 antibody
Anti-Ghf-1 antibody
Anti-POU1F1 antibody
Anti-GHF1 antibody
Anti-PIT1 antibody

Information sourced from Uniprot.org

Citations

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