• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with POU1F1 using POU1F1 Rabbit polyclonal antibody (STJ119954) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-POU1F1 antibody (124-273) (STJ119954)

SKU:
STJ119954

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POU1F1 (124-273) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU1F1
Gene ID: 5449
Uniprot ID: PIT1_HUMAN
Immunogen Region: 124-273
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 124-273 of human POU1F1 (NP_000297.1?).
Immunogen Sequence: MDSPEIRELEKFANEFKVRR IKLGYTQTNVGEALAAVHGS EFSQTTICRFENLQLSFKNA CKLKAILSKWLEEAEQVGAL YNEKVGANERKRKRRTTISI AAKDALERHFGEQNKPSSQE IMRMAEELNLEKEVVRVWFC NRRQREKRVK
Function Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Specifically binds to the consensus sequence 5'-TAAAT-3'. Activates growth hormone and prolactin genes.
Protein Name Pituitary-Specific Positive Transcription Factor 1
Pit-1
Growth Hormone Factor 1
Ghf-1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pituitary-Specific Positive Transcription Factor 1 antibody
Anti-Pit-1 antibody
Anti-Growth Hormone Factor 1 antibody
Anti-Ghf-1 antibody
Anti-POU1F1 antibody
Anti-GHF1 antibody
Anti-PIT1 antibody

Information sourced from Uniprot.org

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