| Short Description : | Rabbit polyclonal PORCN (220-320) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PORCN |
| Gene ID: | 64840 |
| Uniprot ID: | PORCN_HUMAN |
| Immunogen Region: | 220-320 |
| Immunogen Sequence: | PLNGDRLLRNKKRKARWLRA YESAVSFHFSNYFVGFLSEA TATLAGAGFTEEKDHLEWDL TVSKPLNVELPRSMVEVVTS WNLPMSYWLNNYVFKNALRL G |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_982299.1). |
| Tissue Specificity | Isoform 1 is expressed in fetal brain, brain, amygdala, caudate nucleus, cerebellum, hippocampus, pituitary, thalamus, heart, skeletal muscle and testis. Isoform 4 is expressed in amygdala, corpus callosum, hippocampus, spinal cord, kidney, liver, lung, spleen, uterus, testis. Isoform 2 and isoform 3 are expressed in substantia negra, spinal cord, heart and lung. |
| Function | Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1(9Z)), to Wnt proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors. |
| Protein Name | Protein-Serine O-Palmitoleoyltransferase PorcupineProtein Mg61 |
| Database Links | Reactome: R-HSA-3238698Reactome: R-HSA-5340573 |
| Cellular Localisation | Endoplasmic Reticulum MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Protein-Serine O-Palmitoleoyltransferase Porcupine antibodyAnti-Protein Mg61 antibodyAnti-PORCN antibodyAnti-MG61 antibodyAnti-PORC antibodyAnti-PPN antibody |
Information sourced from Uniprot.org
