Western blot analysis of various lysates using PORCN Rabbit polyclonal antibody (STJ114140) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PORCN antibody (220-320) (STJ114140)

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STJ114140
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PORCN
Gene ID: 64840
Uniprot ID: PORCN_HUMAN
Immunogen Region: 220-320
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 220-320 of human PORCN (NP_982299.1).
Immunogen Sequence: PLNGDRLLRNKKRKARWLRA YESAVSFHFSNYFVGFLSEA TATLAGAGFTEEKDHLEWDL TVSKPLNVELPRSMVEVVTS WNLPMSYWLNNYVFKNALRL G
Tissue Specificity Isoform 1 is expressed in fetal brain, brain, amygdala, caudate nucleus, cerebellum, hippocampus, pituitary, thalamus, heart, skeletal muscle and testis. Isoform 4 is expressed in amygdala, corpus callosum, hippocampus, spinal cord, kidney, liver, lung, spleen, uterus, testis. Isoform 2 and isoform 3 are expressed in substantia negra, spinal cord, heart and lung.
Function Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1(9Z)), to Wnt proteins. Serine palmitoleoylation of WNT proteins is required for efficient binding to frizzled receptors.
Protein Name Protein-Serine O-Palmitoleoyltransferase Porcupine
Protein Mg61
Database Links Reactome: R-HSA-3238698
Reactome: R-HSA-5340573
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Protein-Serine O-Palmitoleoyltransferase Porcupine antibody
Anti-Protein Mg61 antibody
Anti-PORCN antibody
Anti-MG61 antibody
Anti-PORC antibody
Anti-PPN antibody

Information sourced from Uniprot.org

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