• Western blot analysis of extracts of various cell lines, using POMP antibody (STJ111551) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using POMP Rabbit polyclonal antibody (STJ111551) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-POMP antibody (1-141) (STJ111551)

SKU:
STJ111551

£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POMP
Gene ID: 51371
Uniprot ID: POMP_HUMAN
Immunogen Region: 1-141
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-141 of human POMP (NP_057016.1).
Immunogen Sequence: MNARGLGSELKDSIPVTELS ASGPFESHDLLRKGFSCVKN ELLPSHPLELSEKNFQLNQD KMNFSTLRNIQGLFAPLKLQ MEFKAVQQVQRLPFLSSSNL SLDVLRGNDETIGFEDILND PSQSEVMGEPHLMVEYKLGL L
Tissue Specificity Strongly expressed from the basal layer to the granular layer of healthy epidermis, whereas in KLICK patients there is a gradual decrease of expression toward the granular layer.
Function Molecular chaperone essential for the assembly of standard proteasomes and immunoproteasomes. Degraded after completion of proteasome maturation. Mediates the association of 20S preproteasome with the endoplasmic reticulum.
Protein Name Proteasome Maturation Protein
Proteassemblin
Protein Ump1 Homolog
Hump1
Voltage-Gated K Channel Beta Subunit 4.1
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Microsome Membrane
Alternative Antibody Names Anti-Proteasome Maturation Protein antibody
Anti-Proteassemblin antibody
Anti-Protein Ump1 Homolog antibody
Anti-Hump1 antibody
Anti-Voltage-Gated K Channel Beta Subunit 4.1 antibody
Anti-POMP antibody
Anti-C13orf12 antibody
Anti-UMP1 antibody
Anti-HSPC014 antibody
Anti-HSPC036 antibody
Anti-PNAS-110 antibody

Information sourced from Uniprot.org

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