Western blot analysis of extracts of various cell lines, using POMGNT1 antibody (STJ111921) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-POMGNT1 antibody (411-660) (STJ111921)

SKU:
STJ111921
£112.50 - £524.50
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POMGNT1
Gene ID: 55624
Uniprot ID: PMGT1_HUMAN
Immunogen Region: 411-660
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human POMGNT1 (NP_060209.3).
Immunogen Sequence: HLLEEDDSLYCISAWNDQGY EHTAEDPALLYRVETMPGLG WVLRRSLYKEELEPKWPTPE KLWDWDMWMRMPEQRRGREC IIPDVSRSYHFGIVGLNMNG YFHEAYFKKHKFNTVPGVQL RNVDSLKKEAYEVEVHRLLS EAEVLDHSKNPCEDSFLPDT EGHTYVAFIRMEKDDDFTTW TQLAKCLHIWDLDVRGNHRG LWRLFRKKNHFLVVGVPASP YSVKKPPSVTPIFLEPPPK
Tissue Specificity Constitutively expressed. An additional weaker band is also detected in spinal cord, lymph node, and trachea. Expressed especially in astrocytes. Also expressed in immature and mature neurons.
Function Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose and does not have MGAT3, MGAT4, MGAT5, MGAT7 or MGAT8 activity.
Protein Name Protein O-Linked-Mannose Beta-1 -2-N-Acetylglucosaminyltransferase 1
Pomgnt1
Udp-Glcnac -Alpha-D-Mannoside Beta-1 -2-N-Acetylglucosaminyltransferase I.2
Gnt I.2
Database Links Reactome: R-HSA-5083628
Reactome: R-HSA-5173105
Cellular Localisation Golgi Apparatus Membrane
Single-Pass Type Ii Membrane Protein
Alternative Antibody Names Anti-Protein O-Linked-Mannose Beta-1 -2-N-Acetylglucosaminyltransferase 1 antibody
Anti-Pomgnt1 antibody
Anti-Udp-Glcnac -Alpha-D-Mannoside Beta-1 -2-N-Acetylglucosaminyltransferase I.2 antibody
Anti-Gnt I.2 antibody
Anti-POMGNT1 antibody
Anti-MGAT1.2 antibody
Anti-UNQ746 antibody
Anti-PRO1475 antibody

Information sourced from Uniprot.org

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