| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-POMGNT1 (411-660) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
POMGNT1 |
| Gene ID: |
55624 |
| Uniprot ID: |
PMGT1_HUMAN |
| Immunogen Region: |
411-660 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 411-660 of human POMGNT1 (NP_060209.3). |
| Immunogen Sequence: |
HLLEEDDSLYCISAWNDQGY EHTAEDPALLYRVETMPGLG WVLRRSLYKEELEPKWPTPE KLWDWDMWMRMPEQRRGREC IIPDVSRSYHFGIVGLNMNG YFHEAYFKKHKFNTVPGVQL RNVDSLKKEAYEVEVHRLLS EAEVLDHSKNPCEDSFLPDT EGHTYVAFIRMEKDDDFTTW TQLAKCLHIWDLDVRGNHRG LWRLFRKKNHFLVVGVPASP YSVKKPPSVTPIFLEPPPK |
| Tissue Specificity | Constitutively expressed. An additional weaker band is also detected in spinal cord, lymph node, and trachea. Expressed especially in astrocytes. Also expressed in immature and mature neurons. |
| Function | Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose and does not have MGAT3, MGAT4, MGAT5, MGAT7 or MGAT8 activity. |
| Protein Name | Protein O-Linked-Mannose Beta-1 -2-N-Acetylglucosaminyltransferase 1Pomgnt1Udp-Glcnac -Alpha-D-Mannoside Beta-1 -2-N-Acetylglucosaminyltransferase I.2Gnt I.2 |
| Database Links | Reactome: R-HSA-5083628Reactome: R-HSA-5173105 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Protein O-Linked-Mannose Beta-1 -2-N-Acetylglucosaminyltransferase 1 antibodyAnti-Pomgnt1 antibodyAnti-Udp-Glcnac -Alpha-D-Mannoside Beta-1 -2-N-Acetylglucosaminyltransferase I.2 antibodyAnti-Gnt I.2 antibodyAnti-POMGNT1 antibodyAnti-MGAT1.2 antibodyAnti-UNQ746 antibodyAnti-PRO1475 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89769/358241/STJ11100834_1__55275.1713122574.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ANKRD52 knockout (KO) 293T cells, using [KO Validated] ANKRD52 Rabbit polyclonal antibody (STJ11100834) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")