| Host: |
Rabbit |
| Applications: |
IHC/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-72 Kda Type Iv Collagenase (30-660) is suitable for use in Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MMP2 |
| Gene ID: |
4313 |
| Uniprot ID: |
MMP2_HUMAN |
| Immunogen Region: |
30-660 |
| Immunogen: |
Recombinant Protein corresponding to a sequence within amino acids 30-660 of human MMP2 (NP_004521.1). |
| Immunogen Sequence: |
APSPIIKFPGDVAPKTDKEL AVQYLNTFYGCPKESCNLFV LKDTLKKMQKFFGLPQTGDL DQNTIETMRKPRCGNPDVAN YNFFPRKPKWDKNQITYRII GYTPDLDPETVDDAFARAFQ VWSDVTPLRFSRIHDGEADI MINFGRWEHGDGYPFDGKDG LLAHAFAPGTGVGGDSHFDD DELWTLGEGQVVRVKYGNAD GEYCKFPFLFNGKEYNSCTD TGRSDGFLWCSTTYNFEKD |
| Tissue Specificity | Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. |
| Post Translational Modifications | Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro. The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3. |
| Function | Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways. |
| Protein Name | 72 Kda Type Iv Collagenase72 Kda GelatinaseGelatinase AMatrix Metalloproteinase-2Mmp-2Tbe-1 Cleaved Into - Pex |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1474228Reactome: R-HSA-1592389Reactome: R-HSA-381426Reactome: R-HSA-3928665Reactome: R-HSA-6785807Reactome: R-HSA-9009391 |
| Cellular Localisation | Isoform 1: SecretedExtracellular SpaceExtracellular MatrixMembraneNucleusColocalizes With Integrin Alphav/Beta3 At The Membrane Surface In Angiogenic Blood Vessels And MelanomasFound In MitochondriaAlong MicrofibrilsAnd In Nuclei Of CardiomyocytesIsoform 2: CytoplasmMitochondrion |
| Alternative Antibody Names | Anti-72 Kda Type Iv Collagenase antibodyAnti-72 Kda Gelatinase antibodyAnti-Gelatinase A antibodyAnti-Matrix Metalloproteinase-2 antibodyAnti-Mmp-2 antibodyAnti-Tbe-1 Cleaved Into - Pex antibodyAnti-MMP2 antibodyAnti-CLG4A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
