Anti-POLR3C antibody (1-270) (STJ118229)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal POLR3C (1-270) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLR3C |
| Gene ID | 10623 |
| Uniprot ID | RPC3_HUMAN |
| Immunogen Region | 1-270 |
| Immunogen Sequence | MTQAEIKLCSLLLQEHFGEI VEKIGVHLIRTGSQPLRVIA HDTGTSLDQVKKALCVLVQH NLVSYQVHKRGVVEYEAQCS RVLRMLRYPRYIYTTKTLYS DTGELIVEELLLNGKLTMSA VVKKVADRLTETMEDGKTMD YAEVSNTFVRLADTHFVQRC PSVPTTENSDPGPPPPAPTL VINEKDMYLVPKLSLIGKGK RRRSSDEDAAGEPKAKRPKY TTDNKEPIPDDGIYWQANL |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human POLR3C (NP_006459.3). |
Additional Info
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Part of POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer, coordinates the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as a nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner. |
| Protein Name | Dna-Directed Rna Polymerase Iii Subunit Rpc3Rna Polymerase Iii Subunit C3Dna-Directed Rna Polymerase Iii Subunit CRna Polymerase Iii 62 Kda SubunitRpc62 |
| Database Links | Reactome: R-HSA-1834949Reactome: R-HSA-73780Reactome: R-HSA-73980Reactome: R-HSA-749476Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase Iii Subunit Rpc3 antibodyAnti-Rna Polymerase Iii Subunit C3 antibodyAnti-Dna-Directed Rna Polymerase Iii Subunit C antibodyAnti-Rna Polymerase Iii 62 Kda Subunit antibodyAnti-Rpc62 antibodyAnti-POLR3C antibody |
Information sourced from Uniprot.org