• Immunohistochemical analysis of paraffin-embedded human brain. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-POLR1E antibody (Internal) (STJ95175)

SKU:
STJ95175

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA-directed RNA polymerase I subunit RPA49 (Internal) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR1E
Gene ID: 64425
Uniprot ID: RPA49_HUMAN
Immunogen Region: Internal
Specificity: POLR1E Polyclonal Antibody detects endogenous levels of POLR1E protein.
Immunogen: Synthesized peptide derived from the Internal region of human POLR1E.
Function DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
Protein Name Dna-Directed Rna Polymerase I Subunit Rpa49
Rna Polymerase I Subunit A49
Dna-Directed Rna Polymerase I Subunit E
Rna Polymerase I-Associated Factor 1
Rna Polymerase I-Associated Factor 53
Database Links Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73863
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerase I Subunit Rpa49 antibody
Anti-Rna Polymerase I Subunit A49 antibody
Anti-Dna-Directed Rna Polymerase I Subunit E antibody
Anti-Rna Polymerase I-Associated Factor 1 antibody
Anti-Rna Polymerase I-Associated Factor 53 antibody
Anti-POLR1E antibody
Anti-PAF53 antibody
Anti-PRAF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance