• Immunohistochemical analysis of paraffin-embedded human brain. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-POLR1E antibody (Internal) (STJ95175)

SKU:
STJ95175

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DNA-directed RNA polymerase I subunit RPA49 (Internal) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POLR1E
Gene ID: 64425
Uniprot ID: RPA49_HUMAN
Immunogen Region: Internal
Specificity: POLR1E Polyclonal Antibody detects endogenous levels of POLR1E protein.
Immunogen: Synthesized peptide derived from the Internal region of human POLR1E.
Function Component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
Protein Name Dna-Directed Rna Polymerase I Subunit Rpa49
Rna Polymerase I Subunit A49
Dna-Directed Rna Polymerase I Subunit E
Rna Polymerase I-Associated Factor 1
Rna Polymerase I-Associated Factor 53
Database Links Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-73762
Reactome: R-HSA-73772
Reactome: R-HSA-73863
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Dna-Directed Rna Polymerase I Subunit Rpa49 antibody
Anti-Rna Polymerase I Subunit A49 antibody
Anti-Dna-Directed Rna Polymerase I Subunit E antibody
Anti-Rna Polymerase I-Associated Factor 1 antibody
Anti-Rna Polymerase I-Associated Factor 53 antibody
Anti-POLR1E antibody
Anti-PAF53 antibody
Anti-PRAF1 antibody

Information sourced from Uniprot.org

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